The TRC shRNA Design Process | Broad Institute
https://www.broadinstitute.org/rnai-consortium/trc-shrna-design-process
WebOverview. We design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse Refseq transcript. The algorithm applies a set of rules, including those derived from the siRNA literature, our cloning scheme, constraints on the synthesis of the oligonucleotides and others.
DA: 59 PA: 16 MOZ Rank: 53