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Frequently Asked Questions

What is needed in DNA replication?

DNA polymerase has 5'-3' activity. All known DNA replication systems require a free 3' hydroxyl group before synthesis can be initiated (note: the DNA template is read in 3' to 5' direction whereas a new strand is synthesized in the 5' to 3' direction-this is often confused).

How many steps are involved in DNA replication?

Three basic steps involved in DNA replication are Initiation, elongation and termination. I. Initiation. Step 1: Binding of DNA around an initiator protein complex DNA-A ATP ~30-40. The DNA B or helicase unwinds ori C (origin of replication) and extends the single stranded region for copying.

What is the goal of DNA replication?

The main purpose of DNA replication is to produce a new daughter cell with a complete set of DNA copied from the parent cell. Replication passes on genetic material to grow new cells and passes on the genome to the daughter cells, ensuring consistency of hereditary information from one cell to another.


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