Colocalization Tools
https://cpb-us-w2.wpmucdn.com/sites.wustl.edu/dist/f/1861/files/2020/10/11-imaris-colocalization-17rwhy5.pdf
Colocalization as a tool for multichannel analysisAcquisition parametersTwo primary kinds of colocalizationStep 1: Region of InterestStep 3: Automatic thresholdSpecial case: Time dependent Auto-ThresholdIllustration>> Tb) AND AND in ROI = co-localized areaPercent ROI MaterialAPPROACH II : distinct objectsManual ThresholdingPercent Material above ThresholdColocalization ResultImaris XtensionsXTension: Spilt Spots into Surface ObjectsXTension: Spots Close To SurfaceImaris has several tools to quantify and visualize colocalization Each addressing specific hypotheses Which method is right for you? Do you want to visualize the colocalization in 3D space? Ultimately what data do you want to export? Colocalization statistics (threshold method) Counts (association/overlap of different puncta) Surface to surface...See more on cpb-us-w2.wpmucdn.com Imaris has several tools to quantify and visualize colocalization Each addressing specific hypotheses Which method is right for you? Do you want to visualize the colocalization in 3D space? Ultimately what data do you want to export? Colocalization statistics (threshold method) Counts (association/overlap of different puncta) Surface to surface... File Size: 982KB Page Count: 27
Imaris has several tools to quantify and visualize colocalization Each addressing specific hypotheses Which method is right for you? Do you want to visualize the colocalization in 3D space? Ultimately what data do you want to export? Colocalization statistics (threshold method) Counts (association/overlap of different puncta) Surface to surface...
File Size: 982KB
Page Count: 27
DA: 94 PA: 43 MOZ Rank: 6