Keyword Analysis & Research: infectious clone

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Keyword CPC PCC Volume Score Length of keyword
infectious clone1.640.387714316
infectious1.07128184710
clone0.340.748781005

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Keyword CPC PCC Volume Score
infectious clone1.870.54638

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  • Development of Infectious Clone of Virus | SpringerLink

    springer.com

    https://link.springer.com/protocol/10.1007/978-1-0716-0334-5_46

    WEBMar 6, 2020 · An infectious clone is a full-length DNA clone from which infectious transcripts can be obtained in vitro or in vivo with a suitable promoter. An infectious clone is the basic need to study functional genomics, replication and expression of viral proteins and in understanding host–virus interactions.

    DA: 86 PA: 61 MOZ Rank: 45

  • An Infectious cDNA Clone of SARS-CoV-2 - PubMed

    nih.gov

    https://pubmed.ncbi.nlm.nih.gov/32289263/

    WEBMay 13, 2020 · RNA transcribed from the full-genome cDNA was highly infectious after electroporation into cells, producing 2.9 × 10 6 plaque-forming unit (PFU)/mL of virus. Compared with a clinical isolate, the infectious-clone-derived SARS-CoV-2 (icSARS-CoV-2) exhibited similar plaque morphology, viral RNA profile, and replication kinetics.

    DA: 53 PA: 47 MOZ Rank: 1

  • Development of Infectious Clone of Virus - Springer Nature

    springernature.com

    https://experiments.springernature.com/articles/10.1007/978-1-0716-0334-5_46

    WEBAn infectious clone is a full-length DNA clone from which infectious transcripts can be obtained in vitro or in vivo with a suitable promoter. An infectious clone is the basic need to study functional genomics, replication and expression of viral proteins and in understanding host–virus interactions.

    DA: 82 PA: 61 MOZ Rank: 12

  • Engineering SARS-CoV-2 using a reverse genetic system - Nature

    nature.com

    https://www.nature.com/articles/s41596-021-00491-8

    WEBJan 29, 2021 · The SARS-CoV-2 infectious complementary DNA (cDNA) clone utilizes an in vitro ligation approach that was pioneered with other coronaviruses, including transmissible gastroenteritis virus...

    DA: 75 PA: 47 MOZ Rank: 97

  • An Infectious cDNA Clone of SARS-CoV-2 - PMC - National …

    nih.gov

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153529/

    WEBMay 5, 2020 · We report the development of a full-length infectious clone and a reporter virus for SARS-CoV-2. One of the key utilities for the reverse genetic system is to facilitate antiviral testing and therapeutic development. The icSARS-CoV-2-mNG reporter virus allows the use of fluorescence as a surrogate readout for viral replication.

    DA: 57 PA: 2 MOZ Rank: 72

  • An Infectious cDNA Clone of SARS-CoV-2 - Cell Press

    cell.com

    https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(20)30231-6

    WEBApr 13, 2020 · An Infectious cDNA Clone of SARS-CoV-2. Highlights. •. A reverse genetic system has been established for SARS-CoV-2. •. Recombinant SARS-CoV-2 replicates as efficiently as the original clinical isolate. •. A stable mNeonGreen reporter SARS-CoV-2 has been developed. •. The mNeonGreen SARS-CoV-2 can be used to screen antiviral …

    DA: 72 PA: 4 MOZ Rank: 42

  • Rapid reconstruction of SARS-CoV-2 using a synthetic genomics ... - Nature

    nature.com

    https://www.nature.com/articles/s41586-020-2294-9

    WEBMay 4, 2020 · The genomes of large RNA viruses, such as those from coronaviruses, are cumbersome to clone and manipulate in Escherichia coli owing to the size and occasional instability of the genome 1, 2, 3....

    DA: 83 PA: 27 MOZ Rank: 44

  • Construction and Rescue of a DNA-Launched DENV2 Infectious Clone

    nih.gov

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959642/

    WEBJan 18, 2023 · In this report, we describe the design and construction of a DNA-launched infectious clone for dengue virus (DENV) serotype 2 strain 16681. An artificial intron was introduced in the nonstructural protein 1 segment of the viral genome to …

    DA: 27 PA: 57 MOZ Rank: 77

  • Coronavirus reverse genetic systems: Infectious clones and replicons

    nih.gov

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727449/

    WEBAug 8, 2014 · The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those …

    DA: 4 PA: 82 MOZ Rank: 43

  • An Infectious cDNA Clone of SARS-CoV-2 - ScienceDirect

    sciencedirect.com

    https://www.sciencedirect.com/science/article/pii/S1931312820302316

    WEBMay 13, 2020 · Using the infectious clone, we can now evaluate the effect of these genetic changes by removing the reported sequences from SARS-CoV-2 and examining their effect on virus replication and S protein processing.

    DA: 80 PA: 39 MOZ Rank: 28