Mueller Hinton Agar (MHA): Composition, Preparation, Uses
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CompositionPreparationQuality ControlWhy Mueller-Hintonagar For AST?Modifications of Muller Hinton AgarLimitations Mueller-Hinton agar1. Weigh appropriate amount of dehydrated Mueller-Hinton agar powder (follow manufacturer’s instructions on bottle) and place in a 2-liter flask. 2. Add 1 liter of distilled water and swirl to disperse powder. 3. Place over a hot plate with a magnetic stirrer(or other heating devices) and … Sheep blood (5%)-supplemented Mueller-Hinton agar1. Prepare 1 liter of MHA as described above. 2. Add 50 ml of sterile defibrinated sheep blood to molten and cooled (48°C) agar. 3. Gently swirl to mix and pour plates as described above. 4. Store prepared plates at 2 to 8°C in tightly sealed packages.
Mueller-Hinton agar1. Weigh appropriate amount of dehydrated Mueller-Hinton agar powder (follow manufacturer’s instructions on bottle) and place in a 2-liter flask. 2. Add 1 liter of distilled water and swirl to disperse powder. 3. Place over a hot plate with a magnetic stirrer(or other heating devices) and …
Sheep blood (5%)-supplemented Mueller-Hinton agar1. Prepare 1 liter of MHA as described above. 2. Add 50 ml of sterile defibrinated sheep blood to molten and cooled (48°C) agar. 3. Gently swirl to mix and pour plates as described above. 4. Store prepared plates at 2 to 8°C in tightly sealed packages.
DA: 51 PA: 35 MOZ Rank: 77