WEBIn eukaryotes, gene fusions use different reporter genes. For example, yeast reporter genes include CUP1, a gene that enables yeast to grow on copper-containing media, URA3, a gene that kills yeast when growing on 5-fluorouracil, and ADE1 and ADE2, two genes that synthesize adenine.
WEBExamples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes cells that express it to glow green under blue or ultraviolet light, [2] the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed [ fr].
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Overview of the reporter genes and reporter mouse models - PMC
WEBApr 19, 2018 · In this review, we describe the types of different reporter genes and reporter mouse models including, random reporter strains, Cre reporter strains and ROSA 26 reporter strains. Collectively, these reporter mouse models have broadened scientific inquires and provided potential strategies for generation of novel reporter animal models …
WEBReporter genes produce detectable signals, making it simple to track and visualize specific gene or protein activity in living cells or organisms through constitutive or inducible expression. Fluorescent proteins, such as GFP, emit colored light when exposed to …
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Bioluminescent Reporters | Reporter Gene Applications | An …
WEBFor example, when tat protein, a transactivating protein in HIV-1, is expressed from one vector in a transfected cell, the activity of different HIV-1 long-terminal repeat (LTR) sequences linked to a reporter gene increases, shown by a corresponding increase of reporter gene protein activity.
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Reporter Gene - an overview | ScienceDirect Topics
WEBAmong the more commonly used conventional reporter genes are those that encode for the following proteins: (a) chloramphenicol acetyltransferase, which transfers radioactive acetyl groups to chloramphenicol; detection by thin-layer chromatography and autoradiography ( Nomura et al., 1997; Zhou et al., 1997 ); (b) β-galactosidase (GAL), which hyd...
WEBCommonly used reporter genes are - GFP (green fluorescent protein gene), lacZ (β- galactosidase gene), RFP (red fluorescent protein gene), and Luc (luciferase gene). The cells expressing GFP glow green when excited by wavelengths of blue to the ultraviolet range, while those expressing RFP glow red when excited by wavelengths of 488 nm or …
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Reporter genes - Latest research and news | Nature
WEBMar 22, 2024 · Reporter genes are genes that enable the detection or measurement of gene expression. They can be fused to regulatory sequences or genes of interest to report expression location or levels. examples
examples
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Reporter Gene - an overview | ScienceDirect Topics
WEBFor example, yeast reporter genes include CUP1, a gene that enables yeast to grow on copper-containing media, URA3, a gene that kills yeast when growing on 5-fluorouracil, and ADE1 and ADE2, two genes that synthesize adenine. ADE1 and ADE2 mutants produce a red pigment when grown on regular media and are easily visualized.
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On the Horizon: Imaging Gene Expression in vivo with Reporter Genes
WEBTypical reporter genes consist of a chimeric gene linking an endogenous or exogenous gene promoter to an enzyme (luciferase or β-galactosidase) or fluorophore (green fluorescent protein [GFP]). examples
