Fast Protein Liquid Chromatography | SpringerLink
https://link.springer.com/protocol/10.1007/978-1-60761-913-0_25
Generally, FPLC chromatography is performed on material that has already been subjected to some preliminary chromatography steps. For anion exchange, the sample must be desalted in buffer A (see Note 3). This may be accomplished by passing it through a column of Sephadex G-25 (45 × 3 cm in diameter, tot… Author: Ashkan Madadlou, Siobhan O’Sullivan, Siobhan O’Sullivan, David Sheehan Publish Year: 2011
Generally, FPLC chromatography is performed on material that has already been subjected to some preliminary chromatography steps. For anion exchange, the sample must be desalted in buffer A (see Note 3). This may be accomplished by passing it through a column of Sephadex G-25 (45 × 3 cm in diameter, tot…
Author: Ashkan Madadlou, Siobhan O’Sullivan, Siobhan O’Sullivan, David Sheehan
Publish Year: 2011
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