Keyword Analysis & Research: steady state spr

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What does SPR stand for?

Principle and Protocol of Surface Plasmon Resonance (SPR) Surface plasmon resonance (SPR) is one of the commonly used technologies for detailed and quantitative studies of protein-protein interactions and determination of their equilibrium and kinetic parameters. SPR provides excellent instrumentation for a label-free,...

How to choose the optimal analyte concentration for SPR?

The analyte concentrations should be confirm before final SPR experiment, which is critical for the experiment. The concentration range should span 10× below the Kd to 10× above the Kd. You should choose the optimal analyte concentration for SPR. Fig.4. Optimize the analyte concentration before SRP experiment

What is meant by the term steady state?

Steady state. When the pulse of analyte continues long enough for the binding to reach steady state, the net rate of binding dR/dt is zero. Since the analyte is continuously added and removed from the system by sample flow, the situation is steady state rather than equilibrium according to the strict definition of the terms...

What is meant by steady state binding?

Steady state. When the pulse of analyte continues long enough for the binding to reach steady state, the net rate of binding dR/dt is zero. Since the analyte is continuously added and removed from the system by sample flow, the situation is steady state rather than equilibrium according to the strict definition of the terms (1).


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